Bidh bacteria innleadaichte a’ tionndadh stalc buntàta gu bhith na phlastaig bith-mhillidh ann an dìreach 24 uair a thìde
Prìomh takeaways
- PHB bioplastic made directly from raw potato starch
- Single fermentation step, 24 hours
- Bacillus subtilis edited with CRISPR-Cas9
- amyQ gene added to encode alpha-amylase
- PHB reached 51.8% of cell dry weight
Carson a tha e cudromach
- Airson luchd-giullachd
- Starch as feedstock for bioplastic is a second outlet for the same raw material that supplies food-grade starch.
- Airson luchd-tasgaidh
- Single-step fermentation is where the cost case sits — the multi-stage process is what has kept PHB expensive.
Geàrr-chunntas 60-diog
Researchers at the University of Barcelona have produced polyhydroxybutyrate (PHB), a biodegradable bioplastic, directly from raw potato starch in a single fermentation step lasting 24 hours — bypassing the multi-stage procedures the process normally requires. The organism is Bacillus subtilis, a safe and widely used industrial bacterium, modified with CRISPR-Cas9 by introducing the amyQ gene encoding alpha-amylase, which lets it break down starch and convert it to PHB in one integrated step. Laboratory results gave 11.3 g/L of biomass and 5.8 g/L of PHB, with the polymer accounting for 51.8% of cell dry weight — figures the authors describe as competitive with commercial standards. Because PHB comes from renewable material and is fully biodegradable, the route is presented as an alternative to petroleum-based plastics that lowers both carbon footprint and persistent waste. These are laboratory yields, not industrial output.
Ann an ceum mòr a dh’ionnsaigh fuasgladh fhaighinn air èiginn truailleadh plastaig na cruinne, tha luchd-rannsachaidh aig Oilthigh Bharcelona air dòigh ùr a leasachadh gus plastaig bith-rùisgte àrd-luachmhor - polyhydroxybutyrate (PHB) - a dhèanamh gu dìreach bho stalc buntàta amh. Tha am pròiseas, a bheir dìreach 24 uair a thìde ann an aon cheum coipeadh, a’ seachnadh nan modhan-obrach ioma-ìre iom-fhillte a bha a dhìth gu traidiseanta. Tha an t-adhartas seo a’ tabhann ro-innleachd gealltanach gus eisimeileachd throm an t-saoghail air connadh fosail airson cinneasachadh plastaig a lughdachadh agus gus an sgudal plastaig maireannach a chruinnicheas ann an lìonadh-fearainn agus cuantan a lughdachadh, agus aig an aon àm a’ lughdachadh sgaoilidhean gasa taigh-glainne co-cheangailte ri losgadh plastaig àbhaisteach.
Aig cridhe na h-innleachdais seo tha am bacteria Bacillus subtilis, meanbh-fhàs-bheairt gnìomhachais sàbhailte agus air a chleachdadh gu farsaing. A’ cleachdadh an inneil deasachaidh gine CRISPR-Cas9, dh’atharraich na luchd-saidheans metabolism a’ bacteria le bhith a’ toirt a-steach a’ ghine amyQ, a bhios a’ còdachadh an einnsein α-amylase. Leig an t-atharrachadh ginteil seo leis a’ bacteria stalc a bhriseadh sìos gu h-èifeachdach agus a thionndadh gu PHB ann an aon cheum amalaichte. Bha toraidhean an obair-lann drùidhteach, a’ toirt a-mach 11.3 g / L de bhith-thomas agus 5.8 g / L de PHB, leis a’ phoilimear a’ dèanamh suas 51.8% de chuideam tioram nan ceallan - figearan a tha gu tur farpaiseach leis na h-inbhean malairteach gnàthach. Leis gu bheil PHB air a thoirt bho ghoireasan ath-nuadhachail agus gu bheil e gu tur bith-ruigsinneach, tha an teicneòlas seo a’ riochdachadh roghainn eile obrachail, càirdeil don àrainneachd an àite plastaig stèidhichte air peatroil, a’ cuideachadh le bhith a’ lughdachadh an dà chuid lorg carboin agus cruinneachadh sgudail leantainneach ann an nàdar.
Geàrr-chunntas Buntàta Cruinneil
Còig prìomh sgeulachdan, prìsean, rabhaidhean galair agus tachartasan. Madainnean làithean seachdaineach, gun sanasachd.
stòran
- University of Barcelona research — named in the article
Ceangailte san stuth seo
- dùthaich
- An Spainn
- Companaidhean
- Oilthigh Barcelona
- Teicneòlasan
- Deasachadh CRISPR-Cas9 air Bacillus subtilis; abairt amyQ / alpha-amylase; coipeadh PHB aon-cheum
- Earrannan
- Uidheam airson pacadhA 'Pacadh
Sgeulachdan co-cheangailte
Bidh luchd-rannsachaidh Eòrpach a’ tionndadh gu bhith a’ cleachdadh impregnation falamh agus earrann bergamot gus sabaid an-aghaidh donnachadh ann am buntàta ùr-gheàrrte
Inneal-seòrsachaidh buntàta àrd-theicneòlais a’ trì-fhillteachadh comas giullachd aig Co-obrachadh Àiteachais Goseong
